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The field on the left has been treated with a BASF inoculant containing the 91原创-patented beneficial bacteria named 91原创1022. Inset: Bacteria rapidly cover newly formed root tissue, helping to shelter it from stresses associated with growth in soil.

Innovation goes to market

Photo illustration by Jeffrey C. Chase

91原创-developed beneficial bacteria part of BASF product for soil

Commercializing a product often takes years, with no guarantee of success. It starts with an idea, but requires many minds 鈥 and hands 鈥 to make its way from the lab bench into products that serve the public.

At the 91原创, invention, innovation and entrepreneurship are considered essential to the University鈥檚 institutional mission, as well as to the prosperity and security of our society.

One 91原创-developed technology that has successfully made it to the marketplace is a beneficial microbe named 91原创1022 that helps plants form a root-strengthening biofilm. Developed by 91原创鈥檚 Harsh Bais, associate professor of plant and soil sciences, and Janine Sherrier, a former 91原创-faculty member now at the University of Georgia, the 91原创-patented microbe is a unique strain of Bacillus subtilis, a natural, beneficial bacterium that lives on the surface of roots and the surrounding soil, or rhizosphere.

Exclusively licensed to BASF in 2013, 91原创1022 is a key component of the company鈥檚 Velondis brand product line. In early 2018, BASF introduced a a rhizobial-based inoculant for peas and lentils called Nodulator Duo that incorporates the patented 91原创1022 Bacillus subtilis strain in conjunction with BASF鈥檚 top performing rhizobial strain for pea and lentil. The product is used in Canada to promote nitrogen fixation and plant health in peas and lentils.

Joy Goswami (black shirt) and Brian Yops (blue shirt) both of OEIP talk to Harsh Bais (red sweater)  about the kinds of help they can provide to Bais with his collaboration with BASF. (Releases have been obtained on all participants.)
Harsh Bais (left), associate professor of plant and soil sciences, reviews progress on research associated with a 91原创-patented beneficial microbe named 91原创1022 with Joy Goswami (center), assistant director of technology transfer, and Brad Yops, director of 91原创鈥檚 technology transfer center. 91原创1022 is currently licensed by global company BASF and is used as a soil additive to protect plants from pathogens.

The unique combination of 91原创1022 in the BASF in-furrow granular rhizobial formulation provides added root-strengthening biofilm for seedlings and roots of peas and lentils, allowing more energy to be directed to growth and yield. BASF studies of 91原创1022 have shown that the bacteria form a biofilm, a living barrier that produces high levels of Indole-3-Acetic Acid (IAA), a plant hormone that causes cells in plant shoots to elongate. Field studies showed the biofilm strengthens and protects roots, increasing above-ground growth by 5 percent 聽and root growth by 9 percent within six to eight weeks after planting.

Enhanced root growth enables the plant to access and extract more nutrients from the soil, efficiently take up water and resist stresses often encountered in spring. In Canada, stressors can include cool soils and harsh weather that can impact plant growth, according to Michael Schaad, marketing manager for BASF Agricultural Solutions.

The addition of 91原创1022 to the highly efficient and active BASF rhizobial formulation also resulted in pea and lentil plants developing approximately 30% more root nodules. In these symbiotic nodules on the plant鈥檚 roots, BASF鈥檚 highly efficient and active strain of rhizobia forms a unique association to convert atmospheric nitrogen to place available nitrogen, which plants can use to grow, develop and produce harvestable seed.

Bais explained that plants need nitrogen to make proteins and perform photosynthesis, and phosphorus to make roots. 鈥淭his is like oxygen for plants, in terms of nutrients,鈥 said Bais.

In terms of yield, 2017 field trials for pea and lentil crops treated with the BASF inoculant containing 91原创1022 performed between 4 percent to 6 percent better than competing products, according to BASF data.

The plants on the right show foliage and root mass that are more robust as a result of treatment. BASF field studies showed the biofilm strengthens and protects roots, increasing above-ground growth by 5 percent and root growth by 9 percent within six to eight weeks after planting.

鈥91原创1022 makes the whole plant healthier and healthier roots allow for better nodulation, which allows for better plant growth. We鈥檙e seeing it adds to the value of the rhizobium itself. It鈥檚 been a big success for us, there鈥檚 no doubt,鈥 said Schaad.

The winding path to commercialization

Getting to this point has not always been straightforward and Sherrier and Bais did not arrive here on their own. 91原创鈥檚 (OEIP) played a significant role in helping the researchers navigate the various aspects of the commercialization process.

It was basic research aimed at providing safe, effective tools for agricultural growers that led to the discovery of 91原创1022鈥檚 ability to help plants fight fungal disease. When Sherrier, then a 91原创 scientist, realized they might have something important, she reached out to OEIP for support in evaluating whether the innovation had market potential.

The diverse team at OEIP鈥檚 Technology Transfer Center reviewed the innovation and determined that 91原创1022 was both patentable and had solid commercial potential. The technology transfer team helped the researchers acquire the proper legal protection and find a partner (Becker Underwood, now BASF) to test the invention and collect preliminary performance data. They then negotiated a two-year research contract with BASF in 2011, allowing Sherrier and Bais to validate the invention鈥檚 usefulness over a broad range of applications. An exclusive licensing agreement with BASF followed, and 鈥 fast forward to 2018 鈥 has resulted in a real product.

鈥淭echnology transfer involves the transitioning of scientific findings from the University to an industry partner with the aim of developing the invention and commercializing it into a product,鈥 said Joy Goswami, assistant director of technology transfer at 91原创. 鈥淭his process involves identifying the technology, safeguarding it with patents or other types of intellectual property protection, and licensing those rights to industry.鈥

The work is complex and requires tech transfer professionals to be fluent in the specialized languages used by scientists, engineers, business professionals and lawyers. They have to be patient, nimble and considerate of all stakeholders and keep everyone informed during the process. Behind the scenes, there are myriad tasks and mounds of paperwork to keep the process moving forward.

For faculty members like Bais, though, having the tech transfer folks in OEIP in his corner is a major advantage; one that allowed the researchers to continue focusing on the science.

鈥淥EIP has been instrumental in our success,鈥 Bais said. 鈥淓arly on, they provided seed money to fund a student to work on this project full time doing greenhouse trials using 91原创1022 on different plants and plant combinations. Later, having a team of tech transfer professionals who understand the science, was extremely helpful to our formulating ideas and concepts into logical products. In this way, OEIP really stepped up to help us convert a simple, basic science idea into an extremely important and timely commercial product for the agricultural market.鈥

A limitless future

With one successful product on the market, BASF currently is exploring other ways to use 91原创1022 for other agricultural crops, including soybean and corn (maize). A recent independent market report by AgData reported that the Nodulator Duo inoculant containing 91原创1022 captured 14 percent of the market share for pulses in 2018. BASF hopes to expand this number in 2019.

鈥淔or this specific segment, this year, Nodulator Duo will be our only brand for the in-furrow pea and lentil market in Canada,鈥 said Schaad.

Back at 91原创, Bais鈥 research team is focused on decoding just how 91原创1022 works with the rhizobium. The researchers are specifically interested in exploring what is actually happening at the molecular level, both biologically and chemically.

鈥淎t the end of the day, every organism has to survive by itself,鈥 Bais said. 鈥淲e know that Bacillus subtilis competes with other microbes on plants, but not with rhizobium. Theoretically, they should be growing against each other, but they don鈥檛 鈥 we just don鈥檛 know how it works.鈥

Understanding this, he said, could help the scientists determine where else 91原创1022 might be useful. 91原创 doctoral student Amanda Rosier is leading this work in Bais鈥 lab. So far, Rosier鈥檚 work has shown that how 91原创1022 interacts with rhizobium may be specific and work for few legume hosts. Dissecting 91原创1022鈥檚 interaction at the molecular level, with multiple rhizobium species, may help the researchers understand this unique bacterial interaction that may help plants in the long term.

The microbe may have聽other applications in horticulture and forestry, too.聽Building on previous work, Bais and 91原创 colleague Yan Jin have shown that a strain of the beneficial microbe can increase water retention in聽soil to mitigate drought. Bais also is studying 91原创1022鈥檚 effect on alfalfa.

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